il 6 elisa kit duo set r d systems  (R&D Systems)

Journal: Radiation Oncology (London, England)

Article Title: Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy

doi: 10.1186/s13014-024-02546-y

Figure Lengend Snippet: Distribution of baseline plasma GDF15 levels. (A) Distribution of individual baseline GDF15 levels for all patients. (B) Comparison of baseline GDF15 levels between the concurrent chemo-radiotherapy (cCRT) and the cCRT + metformin arms (Wilcoxon rank sum test p = 0.44: [median = 963.5pg/ml (475.98-2072.85pg/ml) vs. 1094.2pg/ml (417.23-2444.59pg/ml), respectively]

Article Snippet: Assays were performed using the GDF15 Duo Set ELISA kit (Catalog #DY957, R&D Systems).

Techniques: Comparison

Journal: Radiation Oncology (London, England)

Article Title: Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy

doi: 10.1186/s13014-024-02546-y

Figure Lengend Snippet: Modulation of plasma GDF15 levels by treatments. Levels of GDF15 over the first 26 weeks (6 months) from initiation of cCRT (assessments on weeks 1, 6 and 26 of cCRT or weeks 3, 8 and 26 of metformin treatment). (A) For all patients included in this analysis. (B) For patients based on treatment arm (cCRT: Control, cCRT + Metformin: Metformin). Statistical significance compared to average baseline plasma GDF15 levels, * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical significance compared to average plasma GDF15 level of the Control (cCRT) arm patients at the same timepoint: # p < 0.05, ## p < 0.01

Article Snippet: Assays were performed using the GDF15 Duo Set ELISA kit (Catalog #DY957, R&D Systems).

Techniques: Control

Journal: Radiation Oncology (London, England)

Article Title: Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy

doi: 10.1186/s13014-024-02546-y

Figure Lengend Snippet: Association of plasma GDF15 with tumor volumes. (A) Correlation of baseline GDF15 levels with gross tumor volume (GTV). (B) Correlation of circulating GDF15 levels at the end of week 1 of concurrent chemoradiotherapy (cCRT) with planned tumor volume (PTV).

Article Snippet: Assays were performed using the GDF15 Duo Set ELISA kit (Catalog #DY957, R&D Systems).

Techniques:

Journal: Radiation Oncology (London, England)

Article Title: Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy

doi: 10.1186/s13014-024-02546-y

Figure Lengend Snippet: Kaplan-Meier survival curves of overall (OS) and relapse free (RFS) survival in relation to baseline GDF15 levels for the entire population. A . OS and B . RFS of patients based on baseline GDF15 levels (black line: below the median of the entire population; red dotted line: above the median). C . Overall survival (OS) of patients based on baseline GDF15 levels using the cutoff value of 1465pg/ml reported by Liu et al. (2016)

Article Snippet: Assays were performed using the GDF15 Duo Set ELISA kit (Catalog #DY957, R&D Systems).

Techniques:

Journal: Radiation Oncology (London, England)

Article Title: Growth differentiation factor 15 (GDF15) predicts relapse free and overall survival in unresected locally advanced non-small cell lung cancer treated with chemoradiotherapy

doi: 10.1186/s13014-024-02546-y

Figure Lengend Snippet: Prognostic value of baseline GDF15 compared to tumor volume and stage factors ( Univariate analysis ). Hazard Ratio (HR), confidence interval (95% CI) and p-values are illustrated for relapse free (RFS) and overall (OS) survival

Article Snippet: Assays were performed using the GDF15 Duo Set ELISA kit (Catalog #DY957, R&D Systems).

Techniques:

Journal: Scientific Reports

Article Title: Transcriptomic analysis of melanoma cells reveals an association of α-synuclein with regulation of the inflammatory response

doi: 10.1038/s41598-024-78777-6

Figure Lengend Snippet: Lists some of the genes with the largest Fold change.

Article Snippet: The secretion of cytokines IL-1β and IGFBP5 was quantified using human IL-1β/IL-1F2 Quantikine ELISA Kit (DLB50, RD Systems) and Human IGFBP5 Duo set Quantikine ELISA kit (DY875, RD Systems).

Techniques:

Journal: Scientific Reports

Article Title: Transcriptomic analysis of melanoma cells reveals an association of α-synuclein with regulation of the inflammatory response

doi: 10.1038/s41598-024-78777-6

Figure Lengend Snippet: Genes selected for validation.

Article Snippet: The secretion of cytokines IL-1β and IGFBP5 was quantified using human IL-1β/IL-1F2 Quantikine ELISA Kit (DLB50, RD Systems) and Human IGFBP5 Duo set Quantikine ELISA kit (DY875, RD Systems).

Techniques: Binding Assay, Chemotaxis Assay, Activity Assay

Journal: Scientific Reports

Article Title: Transcriptomic analysis of melanoma cells reveals an association of α-synuclein with regulation of the inflammatory response

doi: 10.1038/s41598-024-78777-6

Figure Lengend Snippet: RT-qPCR validation of RNA-seq data using SK-MEL-28 and WM983B cells. ( A ) Representative western blots of α-syn and α-tubulin in lysates of the control, KO8, and KO9 cells cultured in vitro. ( B ) RT-qPCR analyses of differentially expressed genes in the control and KO cells. Relative mRNA levels in fold-change of IL-1β , IGFBP5 , SAA1 , CXCL8 , CXCL10 , and SNCA normalized to housekeeping gene GAPDH . Relative expression was calculated using the comparative CT method (2 −ΔΔCT ). Triplicates were used per biological sample. Data are mean ± s.d. A one-way ANOVA with Dunnett post hoc test was used to determine p values ( n = 3). * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( C ) Representative western blot of α-syn and α-tubulin in lysates of the control and α-syn KO WM983B cells cultured in vitro. ( D ) RT-qPCR results show gene expression in control and α-syn KO cells on selected transcripts. The comparative CT method (2 −ΔΔCT ) was adopted to calculate the relative mRNA levels of respective genes normalized to the housekeeping gene GAPDH and represented in graphs. Transcripts analyzed are IL-1β , IGFBP5 , SAA1 , CXCL8 , CXCL10 , and SNCA . Data are mean ± s.d. Statistical analysis was performed by using a one-tailed Student’s t-test with unequal variances ( n = 3). * p < 0.05, *** p < 0.001. Uncropped blots are shown in Fig. .

Article Snippet: The secretion of cytokines IL-1β and IGFBP5 was quantified using human IL-1β/IL-1F2 Quantikine ELISA Kit (DLB50, RD Systems) and Human IGFBP5 Duo set Quantikine ELISA kit (DY875, RD Systems).

Techniques: Quantitative RT-PCR, RNA Sequencing Assay, Western Blot, Control, Cell Culture, In Vitro, Expressing, One-tailed Test

Journal: Scientific Reports

Article Title: Transcriptomic analysis of melanoma cells reveals an association of α-synuclein with regulation of the inflammatory response

doi: 10.1038/s41598-024-78777-6

Figure Lengend Snippet: Loss of α-syn expression increases IL-1β and IGFBP-5 secretion in SK-MEL-28 and WM983B cells. ( A ) Representative western blot of α-syn and α-tubulin in lysates of SK-MEL-28 control, SNCA -KO, and SNCA -KI cells. ELISA analysis of secreted IL-1β ( B ) and IGFBP5 ( C ) levels from the culture medium of control, SNCA -KO (KO8 and KO9) and SNCA -KI (KI8 and KI9) cells cultured in vitro. Two technical replicates were used per biological sample. Data are mean ± s.d. P-values were determined using a one-way ANOVA with a Bonferonni correction for multiple comparisons ( n = 3). ELISA analysis of secreted IL-1β ( D ) and IGFBP5 ( E ) levels from the culture medium of WM983B control, SNCA - KO cells cultured in vitro. Two technical replicates were used per biological sample. Data are mean ± s.d. P-values were determined using a one-tailed Student’s t-test with equal variances ( n = 3). *** p < 0.001, **** p < 0.0001. Uncropped blots are shown in Fig. .

Article Snippet: The secretion of cytokines IL-1β and IGFBP5 was quantified using human IL-1β/IL-1F2 Quantikine ELISA Kit (DLB50, RD Systems) and Human IGFBP5 Duo set Quantikine ELISA kit (DY875, RD Systems).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Cell Culture, In Vitro, One-tailed Test

Journal: iScience

Article Title: Deciphering the role of endothelial granulocyte macrophage-CSF in chronic inflammation associated with HIV

doi: 10.1016/j.isci.2024.110909

Figure Lengend Snippet:

Article Snippet: The GM-CSF levels in endothelial cell culture supernatant and in human plasma samples were quantified by using human GM-CSF Duo Set ELISA kit (R&D Systems, Cat# DY215-05).

Techniques: Virus, Microarray, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Fluorescence, Plasmid Preparation, Expressing, Software