human duo set elisa kits (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
94
Structured Review
R&D Systems
human duo set elisa kits
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duo+set+elisa+kit/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duo+set+elisa+kit/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
human duo set elisa kits - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC"
Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
Journal: Redox Biology
doi: 10.1016/j.redox.2026.104172
Figure Legend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control
Figure Legend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot
Related Articles
Cell Culture:Article Title: CNTN4/APP axis of cancer cells and T-cells Article Snippet: After 72 h, dividing cells were evaluated on a FACSCanto II, and data were analyzed using FlowJo software. .. Measure of cytokine secretion The amount of human or mouse IFN-γ or TNF-α in the T cell culture supernatants was determined by ELISA using a Enzyme-linked Immunosorbent Assay:Article Title: CNTN4/APP axis of cancer cells and T-cells Article Snippet: After 72 h, dividing cells were evaluated on a FACSCanto II, and data were analyzed using FlowJo software. .. Measure of cytokine secretion The amount of human or mouse IFN-γ or TNF-α in the T cell culture supernatants was determined by ELISA using a Article Title: Extracellular vesicles in sepsis plasma mediate neuronal inflammation in the brain through miRNAs and innate immune signaling. Article Snippet: .. Following treatment, the supernatant was collected and ELISAs for CXCL2, TNFα, and IL-6 ( Article Title: Modulation of tumor immune microenvironment by TAS-115, a multi-receptor tyrosine kinase inhibitor, promotes antitumor immunity and contributes anti-PD-1 antibody therapy. Article Snippet: .. The concentrations of IFNγ and IL-2 in culture supernatants were determined using Article Title: Fcγ-Receptor-Independent Controlled Activation of CD40 Canonical Signaling by Novel Therapeutic Antibodies for Cancer Therapy. Article Snippet: Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions. .. IL-12p40 cytokine release was quantified using the Article Title: Reduced IFNL1 and/or IFNL2, but not IFNL3 is associated with worse outcome in patients with COVID-19. Article Snippet: © The Author(s) 2024.. Published by Oxford University Press on behalf of the British Society for Immunology.. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Article Title: Chronic Heat Exposure Modulates Innate and Adaptive Immune Responses in Firefighters Article Snippet: .. Plasma derived from the K2EDTA-containing blood was used for measuring the heat stress marker HSP70 using a Article Title: Hyperbaric oxygen augments susceptibility to C. difficile infection by impairing gut microbiota ability to stimulate the HIF-1α-IL-22 axis in ILC3. Article Snippet: Approximately 100 mg of colon tissue was harvested and homogenized in PBS containing protease inhibitors (Thermo Fisher Scientific). .. Samples were centrifuged for 10 min at 2000 × g, and supernatants were used for quantifying TNF-α, IL-6, IL-1β, IL-10, KC (Cxcl1), and MIP-2 (Cxcl2) using the other:Article Title: Long-term histone lactylation connects metabolic and epigenetic rewiring in innate immune memory. Article Snippet: Article Long-term histone lactylation connects metabolic and epigenetic rewiring in innate immune memory Clinical Proteomics:Article Title: Reduced IFNL1 and/or IFNL2, but not IFNL3 is associated with worse outcome in patients with COVID-19. Article Snippet: © The Author(s) 2024.. Published by Oxford University Press on behalf of the British Society for Immunology.. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Article Title: Chronic Heat Exposure Modulates Innate and Adaptive Immune Responses in Firefighters Article Snippet: .. Plasma derived from the K2EDTA-containing blood was used for measuring the heat stress marker HSP70 using a Derivative Assay:Article Title: Chronic Heat Exposure Modulates Innate and Adaptive Immune Responses in Firefighters Article Snippet: .. Plasma derived from the K2EDTA-containing blood was used for measuring the heat stress marker HSP70 using a Marker:Article Title: Chronic Heat Exposure Modulates Innate and Adaptive Immune Responses in Firefighters Article Snippet: .. Plasma derived from the K2EDTA-containing blood was used for measuring the heat stress marker HSP70 using a |
